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The 8 most recent public experiments

Katherine J. Jeppe 1_2, Suzanne Ftouni1_2, Brunda Nijagal4, Leilah K. Grant1_2_3, Steven W. Lockley1_2_3, Shantha M. W. Rajaratnam1_2_3, Andrew J. K. Phillips1, Malcolm J. McConville4, Dedreia Tull4 and Clare Anderson4  

1 School of Psychological Sciences and Turner Institute for Brain and Mental Health, Monash University, Melbourne, Australia. 2 Cooperative Research Centre for Alertness, Safety and Productivity, Melbourne, Australia 3 Division of Sleep and Circadian Disorders, Departments of Medicine and Neurology, Brigham and Women’s Hospital, Boston, USA; Division of Sleep Medicine, Harvard Medical School, Boston, USA 4 Metabolomics Australia, Bio21 Molecular Science and Biotechnology Institute, Parkville, Australia 5 Centre for Human Brain Health, School of Psychology, University of Birmingham, Edgbaston, UK

A.B. Nunez-Nescolarde, ... Chris Barlow, et al  

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Sarah A. Marshall, Remy B. Young, Jessica M. Lewis, Emily L. Rutten, Jodee Gould, Christopher K. Barlow, Cristina Giogha, Vanessa R. Marcelino, Neville Fields, Elizabeth L. Hartland, Nichollas E. Scott, Samuel C. Forster and Emily L. Gulliver  

Metabolomics LC-MS data supporting the publication: The broccoli-derived antioxidant sulforaphane changes the growth of gastrointestinal microbiota, allowing for the production of anti-inflammatory metabolites. Sarah A. Marshall (1), Remy B. Young (2,3), Jessica M. Lewis (4), Emily L. Rutten (2,3), Jodee Gould (2,3), Christopher K. Barlow (5), Cristina Giogha (2,3), Vanessa R. Marcelino (2,3), Neville Fields (1), Elizabeth L. Hartland (2,3), Nichollas E. Scott (4), Samuel C. Forster (2,3) and Emily L. Gulliver (2,3) Correspondence: Affiliations: (1) The Ritchie Centre, Department of Obstetrics and Gynaecology, School of Clinical Sciences, Monash University, Clayton, Victoria, Australia (2) Centre of Innate Immunity and Infectious Disease, Hudson Institute of Medical Research, Clayton, Victoria, Australia (3) Department of Molecular and Translational Sciences, Monash University, Clayton, Victoria, 3800, Australia (4) Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, Victoria, Australia (5) Monash Proteomics & Metabolomics Facility, Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia

Instrument: MPMF_Eclipse User folder name: Uploaded from: THERMO-ER2ATNP3:D:\MyData\\S22_0026

Instrument: MPMF_Exploris User folder name: Uploaded from: 1W3NB93:D:\MyData\\P22_0531

Anna Harutyunyan, Debbie Chong, Rui Li, Anup D. Shah, Zahra Ali, Cheng Huang, Christopher K. Barlow, Piero Perucca, Terence J. O’Brien, Nigel C. Jones, Ralf B. Schittenhelm, Alison Anderson, Pablo M. Casillas-Espinosa  

Metabolomics LC-MS data supporting the publication: An Integrated Multi-Omic Network Analysis Identifies Seizure-Associated Dysregulated Pathways in the GAERS Model of Absence Epilepsy, 2022, International Journal of Molecular Sciences Anna Harutyunyan (1), Debbie Chong (2), Rui Li, Anup D. Shah (3), Zahra Ali (2), Cheng Huang (3), Christopher K. Barlow (3), Piero Perucca (2,4,5,6), Terence J. O’Brien (1,2,4), Nigel C. Jones (1,2,4), Ralf B. Schittenhelm (3), Alison Anderson (1,2,4), Pablo M. Casillas-Espinosa (1,2,4) Correspondence: Samples are sorted into 5 directories: GAERS_Cortex: Samples from the somatosensory cortex in Genetic Absence Epilepsy Rats from Strasbourg (GAERS) rats NEC_Cortex: Samples from the somatosensory cortex in non-epileptic control (NEC) rats GAERS_Thalamus: Samples from thalamus in GAERS rats NEC_Thalamus: Samples from thalamus in NEC rats Pooled_Control: Samples prepared by combining a small amount of the experimental samples and used for quality control during the LC-MS analysis

Viola Oorschot, Benjamin W. Lindsey, Jan Kaslin, Georg Ramm  

Data for Identifying endogenous tissue stem cells remains a key challenge in developmental and regenerative biology. To distinguish and molecularly characterise stem cell populations in large heterogeneous tissues, the combination of cytochemical cell markers with ultrastructural morphology is highly beneficial. Here, we realise this through workflows of multi‐resolution immuno‐correlative light and electron microscopy (iCLEM) methodologies. Taking advantage of the antigenicity preservation of the Tokuyasu technique, we have established robust protocols and workflows and provide a side‐ by‐side comparison of iCLEM used in combination with scanning EM (SEM), scanning TEM (STEM), or transmission EM (TEM). Evaluation of the applications and advantages of each method highlights their practicality for the identification, quantification, and characterization of heterogeneous cell populations in small organisms, organs, or tissues in healthy and diseased states. The iCLEM techniques are broadly applicable and can use either genetically encoded or cytochemical markers on plant, animal and human tissues. We demonstrate how these protocols are particularly suited for investigating neural stem and progenitor cell populations of the vertebrate nervous system.

Flavia Santamaria, Christopher K. Barlow, Rolf Schlagloth, Rupert Palme, Ralf B. Schittenhelm, Edith Klobetz-Rassam, Joerg Henning  

Mass spectrometry data supporting the publication: Characterisation of koala (Phascolarctos cinereus) faecal cortisol metabolites using liquid chromatography-mass spectrometry and enzyme immunoassays. Flavia Santamaria et al., Metabolites, 2021,