Store.Monash Data Store

Metabolomics LC-MS data supporting the publication: The broccoli-derived antioxidant sulforaphane changes the growth of gastrointestinal microbiota, allowing for the production of anti-inflammatory metabolites. Sarah A. Marshall (1), Remy B. Young (2,3), Jessica M. Lewis (4), Emily L. Rutten (2,3), Jodee Gould (2,3), Christopher K. Barlow (5), Cristina Giogha (2,3), Vanessa R. Marcelino (2,3), Neville Fields (1), Elizabeth L. Hartland (2,3), Nichollas E. Scott (4), Samuel C. Forster (2,3) and Emily L. Gulliver (2,3) Correspondence: Emily.gulliver@hudson.org.au Affiliations: (1) The Ritchie Centre, Department of Obstetrics and Gynaecology, School of Clinical Sciences, Monash University, Clayton, Victoria, Australia (2) Centre of Innate Immunity and Infectious Disease, Hudson Institute of Medical Research, Clayton, Victoria, Australia (3) Department of Molecular and Translational Sciences, Monash University, Clayton, Victoria, 3800, Australia (4) Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, Victoria, Australia (5) Monash Proteomics & Metabolomics Facility, Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia

Instrument: MPMF_Eclipse User folder name: Yunjian.Wu@monash.edu Uploaded from: THERMO-ER2ATNP3:D:\MyData\Yunjian.Wu@monash.edu\S22_0026

Instrument: MPMF_Exploris User folder name: Yunjian.Wu@monash.edu Uploaded from: 1W3NB93:D:\MyData\Yunjian.Wu@monash.edu\P22_0531

Metabolomics LC-MS data supporting the publication: An Integrated Multi-Omic Network Analysis Identifies Seizure-Associated Dysregulated Pathways in the GAERS Model of Absence Epilepsy, 2022, International Journal of Molecular Sciences Anna Harutyunyan (1), Debbie Chong (2), Rui Li, Anup D. Shah (3), Zahra Ali (2), Cheng Huang (3), Christopher K. Barlow (3), Piero Perucca (2,4,5,6), Terence J. O’Brien (1,2,4), Nigel C. Jones (1,2,4), Ralf B. Schittenhelm (3), Alison Anderson (1,2,4), Pablo M. Casillas-Espinosa (1,2,4) Correspondence: pablo.casillas-espinosa@monash.edu Samples are sorted into 5 directories: GAERS_Cortex: Samples from the somatosensory cortex in Genetic Absence Epilepsy Rats from Strasbourg (GAERS) rats NEC_Cortex: Samples from the somatosensory cortex in non-epileptic control (NEC) rats GAERS_Thalamus: Samples from thalamus in GAERS rats NEC_Thalamus: Samples from thalamus in NEC rats Pooled_Control: Samples prepared by combining a small amount of the experimental samples and used for quality control during the LC-MS analysis

Data for https://doi.org/10.1038/s41598-020-79637-9 Identifying endogenous tissue stem cells remains a key challenge in developmental and regenerative biology. To distinguish and molecularly characterise stem cell populations in large heterogeneous tissues, the combination of cytochemical cell markers with ultrastructural morphology is highly beneficial. Here, we realise this through workflows of multi‐resolution immuno‐correlative light and electron microscopy (iCLEM) methodologies. Taking advantage of the antigenicity preservation of the Tokuyasu technique, we have established robust protocols and workflows and provide a side‐ by‐side comparison of iCLEM used in combination with scanning EM (SEM), scanning TEM (STEM), or transmission EM (TEM). Evaluation of the applications and advantages of each method highlights their practicality for the identification, quantification, and characterization of heterogeneous cell populations in small organisms, organs, or tissues in healthy and diseased states. The iCLEM techniques are broadly applicable and can use either genetically encoded or cytochemical markers on plant, animal and human tissues. We demonstrate how these protocols are particularly suited for investigating neural stem and progenitor cell populations of the vertebrate nervous system.

Mass spectrometry data supporting the publication: Characterisation of koala (Phascolarctos cinereus) faecal cortisol metabolites using liquid chromatography-mass spectrometry and enzyme immunoassays. Flavia Santamaria et al., Metabolites, 2021, https://www.mdpi.com/2218-1989/11/6/393

Mass spectrometry data supporting the publication: Molecular Basis of Unexpected Specificity of ABC Transporter-Associated Substrate-Binding Protein DppA from Helicobacter pylori, Mohammad M. Rahman et al., Journal of Bacteriology, Vol 201, Issue 20, e00400-19. DOI:10.1128/JB.00400-19 A detailed description of the data processing and location of files may be found in the Read Me.

Crystallisation & diffraction experiment details available in PDB:6nvb. If you use this data, please cite: Acta Cryst. (2019) F75, https://doi.org/10.1107/S2053230X19009610 Crystal structure of the inhibitor-free form of the serine protease kallikrein-4 B. T. Riley, D. E. Hoke, S. McGowan & A. M. Buckle.